Abstract
d-amino acids are increasingly recognized as important signaling molecules in the mammalian central nervous system. However, thed-stereoisomer of the amino acid with the fastest spontaneous racemization ratein vitro in vitro, cysteine, has not been examined in mammals. Using chiral high-performance liquid chromatography and a stereospecific luciferase assay, we identify endogenousd-cysteine in the mammalian brain. We identify serine racemase (SR), which generates theN-methyl-d-aspartate (NMDA) glutamate receptor coagonistd-serine, as a candidate biosynthetic enzyme ford-cysteine.d-cysteine is enriched more than 20-fold in the embryonic mouse brain compared with the adult brain.d-cysteine reduces the proliferation of cultured mouse embryonic neural progenitor cells (NPCs) by ∼50%, effects not shared withd-serine orl-cysteine. The antiproliferative effect ofd-cysteine is mediated by the transcription factors FoxO1 and FoxO3a. The selective influence ofd-cysteine on NPC proliferation is reflected in overgrowth and aberrant lamination of the cerebral cortex in neonatal SR knockout mice. Finally, we perform an unbiased screen ford-cysteine–binding proteins in NPCs by immunoprecipitation with ad-cysteine–specific antibody followed by mass spectrometry. This approach identifies myristoylated alanine-rich C-kinase substrate (MARCKS) as a putatived-cysteine–binding protein. Together, these results establish endogenous mammaliand-cysteine and implicate it as a physiologic regulator of NPC homeostasis in the developing brain.
Funder
HHS | NIH | National Institute of Mental Health
Publisher
Proceedings of the National Academy of Sciences
Cited by
44 articles.
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