Development and quantitative analysis of a biosensor based on the Arabidopsis SWEET1 sugar transporter

Abstract

Significance Transporters are the gatekeepers of the cell. Transporters facilitate the exchange of ions and metabolites between cellular and subcellular compartments, thus controlling processes from bacterial chemotaxis to the release of neurotransmitters. In plants, transporters have key roles in the allocation of carbon to nonphotosynthetic organs. Biosensors derived from transporters have been generated to monitor the activity of these proteins within the complex environment of the cell. However, a quantitative framework that reconciles molecular and cellular-level events to help interpret the response of biosensors is still lacking. Here, we created a sugar transporter biosensor and formulated a mathematical model to explain its response. These types of models can help realize multiscale, dynamic simulations of metabolite allocation to guide crop improvement.