Affiliation:
1. Department of Biological Sciences, Columbia University, New York, NY 10027
Abstract
Significance
Tools that afford spatiotemporal control of gene expression are crucial for studying genes and processes in multicellular organisms. Stop cassettes consist of exogenous sequences that interrupt gene expression and flanking site-specific recombinase sites to allow for tissue-specific excision and restoration of function by expression of the cognate recombinase. We describe a stop cassette called a
flexon
, composed of an artificial exon flanked by artificial introns that can be flexibly positioned in a gene. We demonstrate its efficacy in
Caenorhabditis elegans
for lineage-specific control of gene expression and for tissue-specific RNA interference and discuss other potential uses. The Flexon approach should be feasible in any system amenable to site-specific recombination-based methods and applicable to diverse areas including development, neuroscience, and metabolism.
Funder
Office of Extramural Research, National Institutes of Health
Publisher
Proceedings of the National Academy of Sciences
Cited by
8 articles.
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