Discovery of ultrafast myosin, its amino acid sequence, and structural features-Reference-Cited by-同舟云学术

Discovery of ultrafast myosin, its amino acid sequence, and structural features

Published:2022-02-16 Issue:8 Volume:119 Page:
ISSN:0027-8424
Container-title:Proceedings of the National Academy of Sciences
language:en
Short-container-title:Proc. Natl. Acad. Sci. U.S.A.

Author:

Haraguchi Takeshi¹^ORCID,Tamanaha Masanori¹,Suzuki Kano²^ORCID,Yoshimura Kohei¹,Imi Takuma¹^ORCID,Tominaga Motoki³⁴^ORCID,Sakayama Hidetoshi⁵,Nishiyama Tomoaki⁶,Murata Takeshi²⁷⁸^ORCID,Ito Kohji¹⁷^ORCID

Affiliation:

1. Department of Biology, Graduate School of Science, Chiba University, Chiba 263-8522, Japan

2. Department of Chemistry, Graduate School of Science, Chiba University, Chiba 263-8522, Japan

3. Faculty of Education and Integrated Arts and Sciences, Waseda University, Shinjuku-ku, Tokyo 162-8480, Japan

4. Department of Integrative Bioscience and Biomedical Engineering, Graduate School of Science and Engineering, Waseda University, Shinjuku-ku, Tokyo 162-8480, Japan

5. Department of Biology, Graduate School of Science, Kobe University, Nada-ku, Kobe 657-8501, Japan

6. Research Center for Experimental Modeling of Human Disease, Kanazawa University, Kanazawa 920-0934, Japan

7. Membrane Protein Research and Molecular Chirality Research Center, Chiba University, Chiba 263-8522, Japan

8. Structure Biology Research Center, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK), Tsukuba 305-0801, Japan

Abstract

Significance It has been suggested for more than 50 y that the fastest myosin in the biological world with a velocity of 70 μm s −1 exists in the alga Chara , because cytoplasmic streaming with a velocity of 70 μm s −1 occurs in Chara cells. However, a myosin with that velocity has not yet been identified. In this work, we succeeded in cloning a myosin XI with a velocity of 60 μm s −1 , which was measured using a chimeric myosin. We also successfully crystallized myosin XI. Structural comparison of various myosins and mutation experiments of actin-binding regions suggests that the central regions that define the fast movement of Chara myosin XI are the actin-binding sites.

Publisher

Proceedings of the National Academy of Sciences

Subject

Multidisciplinary

Link

https://pnas.org/doi/pdf/10.1073/pnas.2120962119

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