Targeted base editing in the mitochondrial genome of Arabidopsis thaliana

Abstract

Significance The mitochondrial genomes of land plants encode genes for cellular energy production and agriculturally important traits, but modification of the genomes is still difficult. Targeted base editing is one of the best ways to modify genes and intergenic regions and thus understand their functions, without drastically changing genome structure. In this study, we succeeded in creating plantlets of the model plant Arabidopsis thaliana , in which all of the many copies of the mitochondrial genomes in each cell had a targeted C:G base pair converted to a T:A pair. Introduced mutations were stably inherited by the next generation. This method will help to unravel the mysteries of plant mitochondrial genomes and may also serve as a basis for increasing crop yields.