A VDAC1-mediated NEET protein chain transfers [2Fe-2S] clusters between the mitochondria and the cytosol and impacts mitochondrial dynamics-Reference-Cited by-同舟云学术

A VDAC1-mediated NEET protein chain transfers [2Fe-2S] clusters between the mitochondria and the cytosol and impacts mitochondrial dynamics

Published:2022-02-08 Issue:7 Volume:119 Page:
ISSN:0027-8424
Container-title:Proceedings of the National Academy of Sciences
language:en
Short-container-title:Proc. Natl. Acad. Sci. U.S.A.

Author:

Karmi Ola¹²³,Marjault Henri-Baptiste¹²,Bai Fang⁴⁵⁶⁷⁸^ORCID,Roy Susmita⁹^ORCID,Sohn Yang-Sung¹²,Darash Yahana Merav¹²,Morcos Faruck¹⁰¹¹^ORCID,Ioannidis Konstantinos¹²¹²,Nahmias Yaakov¹²¹²,Jennings Patricia A.¹³,Mittler Ron³^ORCID,Onuchic José N.⁴⁵⁶^ORCID,Nechushtai Rachel¹²

Affiliation:

1. The Alexander Silberman Institute of Life Science, Faculty of Science and Mathematics, The Hebrew University of Jerusalem, Jerusalem 91904, Israel

2. The Wolfson Centre for Applied Structural Biology, Faculty of Science and Mathematics, The Hebrew University of Jerusalem, Jerusalem 91904, Israel

3. Department of Surgery, University of Missouri School of Medicine, University of Missouri, Columbia, MO 65211

4. Center for Theoretical Biological Physics, Department of Physics and Astronomy, Rice University, Houston, TX 77005

5. Department of Chemistry, Rice University, Houston, TX 77005

6. Department of Biosciences, Rice University, Houston, TX 77005

7. Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai 201210 China

8. School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China

9. Indian Institute of Science Education and Research Kolkata, West Bengal 741246, India

10. Department of Biological Sciences, University of Texas at Dallas, Richardson, TX 75080

11. Department of Bioengineering, University of Texas at Dallas, Richardson, TX 75080

12. Alexander Grass Center for Bioengineering, The Hebrew University of Jerusalem, Jerusalem 91904, Israel

13. Department of Chemistry & Biochemistry, University of California San Diego, La Jolla, CA 92093

Abstract

Significance Here we address the important question of cross-talk between the mitochondria and cytosol. We show that the inner mitochondrial protein, MiNT, interacts with a protein on the outer mitochondrial membrane (mNT). This interaction occurs within the major outer membrane protein VDAC1. Inside the inner space of VDAC1, MiNT transfers its [2Fe-2S] clusters to mNT, which was shown to be a [2Fe-2S] cluster donor protein that donates its cluster(s) to apo-acceptor proteins residing in the cytosol. Hence, we suggest a pathway for transferring [2Fe-2S] clusters from inside the mitochondria to the cytosol.

Funder

National Science Foundation

Welch Foundation

Publisher

Proceedings of the National Academy of Sciences

Subject

Multidisciplinary

Link

https://pnas.org/doi/pdf/10.1073/pnas.2121491119

Reference58 articles.

1. Iron–sulfur cluster biogenesis and trafficking in mitochondria

2. Structure and functional dynamics of the mitochondrial Fe/S cluster synthesis complex