Hatching is modulated by microRNA-378a-3p derived from extracellular vesicles secreted by blastocysts-Reference-Cited by-同舟云学术

Hatching is modulated by microRNA-378a-3p derived from extracellular vesicles secreted by blastocysts

Published:2022-03-17 Issue:12 Volume:119 Page:
ISSN:0027-8424
Container-title:Proceedings of the National Academy of Sciences
language:en
Short-container-title:Proc. Natl. Acad. Sci. U.S.A.

Author:

Pavani Krishna Chaitanya¹²^ORCID,Meese Tim³,Pascottini Osvaldo Bogado¹⁴^ORCID,Guan XueFeng⁵^ORCID,Lin Xiaoyuan⁵,Peelman Luc⁵,Hamacher Joachim⁶,Van Nieuwerburgh Filip³^ORCID,Deforce Dieter³^ORCID,Boel Annekatrien⁷^ORCID,Heindryckx Björn⁷,Tilleman Kelly²,Van Soom Ann¹^ORCID,Gadella Bart M.⁸^ORCID,Hendrix An⁹¹⁰,Smits Katrien¹^ORCID

Affiliation:

1. Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, University of Ghent, B-9820 Merelbeke, Belgium

2. Department for Reproductive Medicine, Ghent University Hospital, 9000 Gent, Belgium

3. Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Ghent University, B-9000 Ghent, Belgium

4. Department of Veterinary Sciences, Gamete Research Center, University of Antwerp, 2610 Antwerp, Belgium

5. Department of Nutrition, Genetics and Ethology, Faculty of Veterinary Medicine, Ghent University, B-9000 Ghent, Belgium

6. Institute of Crop Science and Resource Conservation, Plant Pathology, Rheinische Friedrich-Wilhelms-University of Bonn, D-53115 Bonn, Germany

7. Ghent-Fertility and Stem Cell Team, Department for Reproductive Medicine, Ghent University Hospital, 9000 Ghent, Belgium

8. Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, 3584 CM Utrecht, The Netherlands

9. Laboratory of Experimental Cancer Research, Department of Human Structure and Repair, Ghent University, B-9000 Ghent, Belgium

10. Cancer Research Institute Ghent, B-9000 Ghent, Belgium

Abstract

Significance Hatching from the zona pellucida is a prerequisite for embryo implantation and is less likely to occur in vitro for reasons unknown. Extracellular vesicles (EVs) are secreted by the embryo into the culture medium. Yet the role that embryonic EVs and their cargo microRNAs (miRNAs) play in blastocyst hatching has not been elucidated, partially due to the difficulties of isolating them from low amounts of culture medium. Here, we optimized EV-miRNA isolation from medium conditioned by individually cultured bovine embryos and subsequently showed that miR-378a-3p, which was up-regulated in EVs secreted by blastocysts, plays a crucial role in promoting blastocyst hatching. This demonstrates the regulatory effect of miR-378-3p on hatching, which is an established embryo quality parameter linked with implantation.

Publisher

Proceedings of the National Academy of Sciences

Subject

Multidisciplinary

Link

https://pnas.org/doi/pdf/10.1073/pnas.2122708119

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