RN7SL1 may be translated under oncogenic conditions-Reference-Cited by-同舟云学术

RN7SL1 may be translated under oncogenic conditions

Published:2024-03-13 Issue:12 Volume:121 Page:
ISSN:0027-8424
Container-title:Proceedings of the National Academy of Sciences
language:en
Short-container-title:Proc. Natl. Acad. Sci. U.S.A.

Author:

Hara Tomoaki¹,Meng Sikun¹,Tsuji Yoshiko¹,Arao Yasuko¹,Saito Yoshiko¹,Sato Hiromichi¹²,Motooka Daisuke³,Uchida Shizuka⁴,Ishii Hideshi¹^ORCID

Affiliation:

1. Department of Medical Data Science, Center of Medical Innovation and Translational Research, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan

2. Department of Gastroenterological Surgery, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan

3. Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan

4. Center for RNA Medicine, Department of Clinical Medicine, Aalborg University, Copenhagen SV DK-2450, Denmark

Abstract

RN7SL1 (RNA component of signal recognition particle 7SL1), a component of the signal recognition particle, is a non-coding RNA possessing a small ORF (smORF). However, whether it is translated into peptides is unknown. Here, we generated the RN7SL1-Green Fluorescent Protein (GFP) gene, in which the smORF of RN7SL1 was replaced by GFP, introduced it into 293T cells, and observed cells emitting GFP fluorescence. Furthermore, RNA-seq of GFP-positive cells revealed that they were in an oncogenic state, suggesting that RN7SL1 smORF may be translated under special conditions.

Funder

Ministry of Education, Culture, Sports, Science and Technology

Publisher

Proceedings of the National Academy of Sciences

Link

https://pnas.org/doi/pdf/10.1073/pnas.2312322121

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