The embryonic node behaves as an instructive stem cell niche for axial elongation

Abstract

Significance Previous studies have suggested that the amniote node (Hensen’s node) contains a small population of self-renewing resident cells whose progeny progressively lay down axial tissues, including notochord and somites. This can only be demonstrated definitively at the level of single cells. Here we ask whether the node is an environment that can confer this behavior on cells that enter it. We challenge single cells in vivo and mRNA-profile these cells to demonstrate that the node can indeed do this, and thus show that the node acts as an instructive niche.