Affiliation:
1. Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, CA 90095-1662
Abstract
Significance
The dorsal axis of the vertebrate
Xenopus
embryo is established by an early Wnt signal generated by a rotation of the cortex of the egg toward the opposite side of the sperm entry point. In this study, we report that lysosomal cathepsin D becomes activated on the dorsal marginal zone of the embryo already at the 64-cell stage, and that this asymmetry is enhanced by increasing Wnt signaling levels. We present experiments showing that lysosome acidification, macropinocytosis, and multivesicular body formation are required for the endogenous dorsal signal provided maternally in the egg. The results indicate that the cell biology of lysosomes plays an essential role in vertebrate development.
Funder
HHS | NIH | National Cancer Institute
Publisher
Proceedings of the National Academy of Sciences
Cited by
11 articles.
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