Author:
BORDMANN G.,FAVRE N.,RUDIN W.
Abstract
The ability of deproteinated malaria exoantigens from Plasmodium
falciparum (Pf-MT) and P. berghei ANKA (PbA-MT)
to activate murine haematopoietic cells was analysed in vitro.
Malaria toxins (MT) of both plasmodium species induced
cell proliferation and the production of IFN-γ in overnight and
long-term (5 days) spleen and bone marrow cultures and
a reduction of the number of TNF-α spot forming cells (SFC).
When added to cells of malaria-experienced animals, MT
decreased the number of IL-4 SPC and increased the number of IL-5 SPC.
However,
the same proliferative and IFN-γ induction properties as in
naive cells were observed. Simultaneous addition of IL-2 and PbA-MT to
spleen
cells inhibited
the proliferation but increased the IFN-γ production usually induced
by
IL-2. Flow cytometric analysis revealed that the
addition of MT triggered an expansion of CD3+ and GR1+
cell populations. Our results suggest that malaria toxins of
different species can induce an immediate and strong proliferation and
a
TH1-type cytokine release by murine cells,
independently of previous in vivo priming.
Publisher
Cambridge University Press (CUP)
Subject
Infectious Diseases,Animal Science and Zoology,Parasitology
Cited by
6 articles.
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