Author:
KUTISOVA K.,KULDA J.,CEPICKA I.,FLEGR J.,KOUDELA B.,TERAS J.,TACHEZY J.
Abstract
To clarify the taxonomy of trichomonads associated with human respiratory diseases, we examined a collection of axenic trichomonad strains isolated from the oral cavity and bronchi of patients from pulmonary diseases clinics in Tallin, Estonia. The oral and bronchial strains were compared mutually as well as with a reference strain of Trichomonas tenax, a common inhabitant of the human oral cavity, and other trichomonad species from humans and animals. Unexpectedly, the morphological studies, as well as DNA sequencing of ITS1-5.8S rRNA-ITS2 regions revealed that the Estonian strains belong to the genus Tetratrichomonas, with a high similarity to the avian species Tetratrichomonas gallinarum. None of the strains belonged to Trichomonas tenax. DNA fingerprinting using the RAPD method separated Estonian strains into 2 distinct groups: ‘bronchial’ consisting of 5 and 2 strains isolated from bronchi and ‘oral’ cavity, respectively, and oral consisting of 3 oral strains. Consistent differences between ‘bronchial’ and ‘oral’ groups were confirmed by analysis of ITS1-5.8S rRNA-ITS2 sequences. Our results have revealed novel trichomonad species of the human oral cavity and bronchi.
Publisher
Cambridge University Press (CUP)
Subject
Infectious Diseases,Animal Science and Zoology,Parasitology
Reference58 articles.
1. Walker, R. L. , Hayes, D. C. , Sawyer, S. J. , Nordhausen, R. W. , Van Hoosear, K. A. and Bondurant, R. H. (2003).Comparison of the 5.8S rRNA gene and internal transcribed spacer regions of trichomonadid protozoa recovered from the bovine preputial cavity.Journal of Veterinary Diagnostic Investigation 15,14–20.
2. Thompson, J. D. , Gibson, T. J. , Plewniak, F. , Jeanmougin, F. and Higgins, D. G. (2000).The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.Nucleic Acids Research 25,4876–4882.
3. Teras, J. K. , Roigas, E. M. , Kazakova, I. I. , Ranne, H. R. , Trapido, L. E. , Sardis, H. J. and Kaal, V. A. (1980).Detection of Trichomonas in the bronchi, sputum and oral cavity in patients with diverse pulmonary abnormalities.Terapeuticheskii Arkhiv 52,123–125.In Russian, English summary.
4. Teras, J. , Kumm, R. and Kallas, E. V. (1972).Intraspecific variations of Trichomonas tenax . In 2nd Congress of Epidemiology, Microbiology and Hygiene , pp.370–372.Tallin.In Russian.
5. Effect of Certain Laboratory Procedures on Virulence of the Jones' Barn Strain of Trichomonas gallinae for Pigeons
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