DNA base composition of filarial nematodes

Abstract

SUMMARYWe have determined the molar content of guanine + cytosine (GC content) of DNA of the filarial nematode (Brugia malayi, Brugia pahangiandDirofilaria imitis) and of the free-living soil nematodesCaenorhabditis elegansand have analysed the DNA for the presence of methylcytosine. Two independent methods, thermal denaturation and direct analysis of base content by HPLC following enzymatic hydrolysis, reveal that the GC content of filarial nematodes is 26–28%. We have been unable to find methylcytosine in the DNA ofB. malayi.