Thermophilic bacteria present in a sample from Fumarole Bay, Deception Island

Abstract

AbstractDeception Island, an active stratovolcano located in the South Shetland Islands, Antarctica, provides excellent conditions for the thermophilic bacteria growth because of high ground temperatures in specific areas, such as Fumarole Bay where the temperatures are above the mesophilic range. Denaturing Gradient Gel Electrophoresis (DGGE) was used with the 16S ribosomal gene to analyse cultures of thermophilic bacteria from a soil sample taken from Fumarole Bay. Nine bands were sequenced and analysed from DGGE and they indicated the presence of bacteria from the generaGeobacillus,Bacillus,Brevibacillus,Thermusand uncultured sulphate reducing bacteria. Some of which have been reported in other Antarctic geothermal sites.Geobacillus,BacillusandBrevibacillusgenera were successfully cultivated in an enriched medium. A pure culture of one thermophilicGeobacillusbacterium was obtained closely related toGeobacillus jurassicus.