Duddingtonia flagrans,Monacrosporium thaumasiumandPochonia chlamydosporiaas possible biological control agentsof Oxyuris equiandAustroxyuris finlaysoni

Abstract

AbstractThe action of four fungal isolates of the speciesDuddingtonia flagrans(AC001),Monacrosporium thaumasium(NF34a) andPochonia chlamydosporia(VC1 and VC4) on eggs ofOxyuris equiandAustroxyuris finlaysoniwas evaluated in two assays (A and B). Eggs ofO. equi(Test A) andA. finlaysoni(Test B) were plated on Petri dishes with 2% water-agar with grown fungal isolates and control without fungus. After 5, 10 and 15 days, 100 eggs were collected and classified according to the following parameters: type 1 effect, physiological and biochemical effect without morphological damage to the eggshell; type 2 effect, lytic effect with morphological alteration of the eggshell and embryo; and type 3 effect, lytic effect with morphological alteration of the eggshell and embryo, hyphal penetration and internal egg colonization.Pochonia chlamydosporiaisolates VC1 and VC4 showed ovicidal activity for type 1, 2 and 3 effects on eggs ofO. equiand eggs ofA. finlaysoni.In vitroassays A and B showed thatP. chlamydosporiahad a negative influence on eggs ofO. equiandA. finlaysoniand can be considered as a potential biological control agent of nematodes.