On the identification of some species of Trichostrongylus

Abstract

A method is described by means of which trichostrongylid spicules are separated from the surrounding soft tissues and their examination made easier. NaOH and KOH are the most satisfactory substances for this purpose, though both substances eventually act on the chitin of the spicules themselves, causing distortion. The reaction takes place at a temperature of 37°C. with reasonable speed. Spicules of Ostertagia can also be freed satisfactorily by this method but those of Nematodirus are quickly affected by the alkali, with consequent distortion.