Enzymatic Pretreatment for Extraction of Starch Encapsulated Pesticides from Soils

Author:

Wienhold Brian J.,Gish Timothy J.

Abstract

An enzymatic (amylase) pretreatment was developed that allows quantitative recovery of the parent compound from soil samples receiving the pesticide in starch-encapsulated formulation. The pretreatment involves adding amylase, dissolved in a phosphate buffer solution, to soil samples and incubating the resulting slurry at 50 C for 1 h. Efficiency of this pretreatment was tested on two soils, a silty clay and a loamy sand, receiving atrazine and alachlor in the starch-encapsulated formulation. The inexpensive method adds approximately 1 h to the extraction procedure and a twofold increase in percentage recovery of starch-encapsulated atrazine. The enzyme pretreatment is not necessary for quantitative recovery of pesticides that have rapid rates of release such as alachlor. The method will be useful for studying starch encapsulation pesticide behavior in the soil environment.

Publisher

Cambridge University Press (CUP)

Subject

Plant Science,Agronomy and Crop Science

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