Application of immunogold/quantitative x-ray microanalysis to study infectious hematopoietic necrosis virus

Abstract

Monoclonal antibody staining followed by immunogold labeling and energy dispersive x-ray microanalysis was utilized to detect the presence of the nucleoprotein (N) and glycoprotein (G) of infectious hematopoietic necrosis virus (IHNV) on the surface of cultured coho salmon embryo (CSE-119) and rainbow trout heart (RTH) cell lines. The objectives of this study were to first, demonstrate that anti-G and anti-N monoclonal antibodies labeled with streptavidin gold would identify virus bound to the surface of cultured fish cells. Secondly, we sought to demonstrate the quantities of colloidal gold particles associated with the surface region of IHNV infected cells at 1, 4, 6, 8, 14, 24, and 30 hours.