Genetic analysis of actidione-resistant mutants in the MyxomycetePhysarum polycephalum, Schw.

Abstract

1. Improved methods for the culture and preservation ofPhysarum polycephalumamoebae have been developed.2. Strains of amoebae have been isolated which are stably resistant to 4, 8 or 16 μg./ml. actidione.3. Sensitivity vs. resistance to 4 μg./ml. actidione is probably controlled by a single pair of alleles at a locus unlinked to the mating-type locus.4. Plasmodia homozygous or heterozygous for the allele determining resistance at this locus are no more resistant to actidione than plasmodia homozygous for the allele determining sensitivity.