Effect of antibiotic on survival and development ofSpodoptera litura(Lepidoptera: Noctuidae) and its gut microbial diversity

Abstract

AbstractAddition of antibiotics to artificial diets of insects is a key component in the rearing of insects in the laboratory. In the present study an antimicrobial agent, streptomycin sulphate was tested for its influence on survival and fitness ofSpodoptera litura(Fabricus) (Lepidoptera: Noctuidae) as well as its gut microbial diversity. The antibiotic did not adversely affect the survival ofS. litura.Faster growth of larvae was recorded on diet amended with different concentrations of streptomycin sulphate (0.03, 0.07 and 0.15%) as compared to diet without streptomycin sulphate. The overall activity of various digestives enzymes increased on S+ diet while the activity of detoxifying enzymes significantly decreased. In addition, alteration in microbial diversity was found in the gut ofS. lituralarvae fed on diet supplemented with antibiotic (S+) and without antibiotic (S−).