Distinguishing the females ofPhlebotomus (Synphlebotomus) martiniandP. (S.) celiae(Diptera: Phlebotominae), vectors of visceral leishmaniasis in southern Ethiopia

Abstract

AbstractThe inability to distinguish the females of East AfricanSynphlebotomusspp. (P. martiniParrot,P. vansomerenaeHeisch, Guggisberg & Teesdale andP. celiaeMinter) was a major constraint on epidemiological studies of visceral leishma niasis (VL) in the region. An intensive morphometric study of two sympatricSynphlebotomusspp. (P. martiniandP. celiae) in a VL focus of southern Ethiopia based on both isofemale broods and wild-caught parent specimens (identified by their male progenies) showed that laboratory-bred females were separable by eight morphological characters, but only two (labrum length and labrum length/wing length ratio) successfully distinguished the wild-caught parent females of the two species; the latter were used to identify wild-caughtSynphlebotomusfemales from southern Ethiopia (Aba Roba) during subsequent epidemiological studies. A further difference between the two species was seen in the length of the spermathecal ducts.