Molecular typing ofSalmonella entericaserovars Enteritidis, Corvallis, Anatum and Typhimurium from food and human stool samples in Tunisia, 2001–2004

Abstract

SUMMARYDuring the period from 2001 to 2004, a total of 72 isolates ofSalmonella entericaserovars: Anatum (n=40), Enteritidis (n=18), Corvallis (n=8), and Typhimurium (n=6), of various origins (mainly food and diarrhoeagenic stool samples), were collected and further characterized by antibiotic resistance, plasmid analysis, and pulsed-field gel electrophoresis (PFGE). Forty-five isolates presented multidrug resistance to antibiotics. Among which oneS. entericaserovar Anatum isolate was resistant to 11 antibiotics, and oneS. entericaserovar Typhimurium DT104 isolate was resistant to eight antibiotics. Plasmid profiling identified eight plasmid profiles (with 1–5 plasmids) among the isolates, of which one plasmid profile (P01) was predominant.XbaI PFGE analysis revealed the presence of a predominant clone of the four studiedSalmonellaserovars circulating in Tunisia throughout the years 2001–2004.