Babesiaparasites develop and are transmitted by the non-vector soft tickOrnithodoros moubata(Acari: Argasidae)

Abstract

Ornithodoros moubataticks were fed on blood infected withBabesia equi. However, the parasites were quickly cleared as evidenced by the disappearance ofB. equi-specific ribosomal RNA from the ticks. We hypothesized that if theBabesiaparasite can escape midgut-associated barriers a non-vector tick can become infected withBabesia. To test this hypothesis,B. equiparasite-infected blood fromin vitroculture was injected into the haemocoel of ticks.B. equi-specific rRNA was surprisingly detected 45 days after injection even in the eggs.Babesia-free dogs were infested withO. moubataticks that were infected by inoculation withB. gibsoni-infected red blood cells. Parasitaemia and antibody production against Bg-TRAP ofB. gibsoniincreased gradually. These results indicate thatO. moubatamay be a useful vector model forBabesiaparasites and also a very important tool for studies on tick immunity againstBabesiaparasites and tick-Babesiainteractions.