An analysis of the proteinases ofTrichomonas vaginalisby polyacrylamide gel electrophoresis

Abstract

SUMMARYThe proteinases ofTrichomonas vaginalishave been analysed by electrophoresis in polyacrylamide gels containing denatured haemoglobin. Seven bands of activity were detected indicating multiple proteinases. All of the enzymes were stimulated by 1 mM dithiothreitol and had inhibitor sensitivities characteristic of cysteine proteinases. The enzymes differed significantly, however, with respect to pH optima and relative sensitivities to inhibitors.