Author:
Sawada Yosuke,Fujii Reiko,Igami Ikuo,Kawai Atsushi,Kamiki Teruo,Niwa Makoto
Abstract
SUMMARYThe microporous polyethylene hollow fibre membrane is capable of adsorbing small-sized lipopolysaccharides (LPS) prepared by sonication dispersion, column chromatography on Sephadex G-75 and filtration through a filter membrane with a nominal pore size of 0·025 μg. Small-sized LPS had a one-thousandth of endotoxin activity as compared to intact LPS, when determined by the Synthetic Chromogenic Substrate method of LAL with a specific endotoxin activity of 73·7 ng/μg LPS. Fluorescent microscopy of fluorescein conjugated LPS on a microporous polyethylene hollow fibre showed that fiuorescein-LPS was adsorbed through the entire depth of the membrane texture. Accordingly the adsorption capacity of the filter for small-sized LPS was determined as 1·65 mg LPS/3·68 m2surface/116 mg fibre/module.
Publisher
Cambridge University Press (CUP)
Subject
Public Health, Environmental and Occupational Health,Immunology
Reference27 articles.
1. Conditions that affect the colorimetric analysis of lipopolysaccharides from Escherichia coli and Treponema pallidum;Zey;Applied Microbiology,1973
2. The reassociation of lipopoly-saccharide, phospholipid and transferase enzyme of the bacterial cell envelop. Isolation of binary and ternary complexes;Weiskk;The Journal of Biological Chemistry,1908
3. Study of microassay of pyrogen in vitro(1). Application of chromogenic assay method (Synthetic substrate method);Usami;Annual Report of Tokyo Metropolitan Research Laboratory of Public Health,1981
4. Enhancement of Endotoxicity and Reactivity with Carbocyanine Dye by Sonication of Lipopolysaccharide
5. The dispersion of gram-negative lipopolysaccharide by deoxycholate;Shands;The Journal of Biological Chemistry,1980
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献