Qualitative hepatitis C virus RNA assay identifies active infection with sufficient viral load for treatment among Phetchabun residents in Thailand

Author:

Pratedrat Pornpitra,Nilyanimit Pornjarim,Wasitthankasem Rujipat,Posuwan Nawarat,Auphimai Chompoonut,Hansoongnern Payuda,Pimsing Napaporn,Ngamnimit Saranya,Thongmai Chaiwat,Phaengkha Wijittra,Wanlapakorn Nasamon,Vongpunsawad Sompong,Poovorawan YongORCID

Abstract

The World Health Organization envisions the elimination of viral hepatitis by 2030 through reducing prevalence and transmission, increasing diagnostic screening, and expanding treatment coverage. Efforts to micro-eliminate hepatitis in Phetchabun province in Thailand, a region where the prevalence of hepatitis C virus (HCV) infection and liver cancer is higher than elsewhere in the country, began with evaluating the province-wide burden of HCV. Here, we describe a feasibility study to assess active HCV infection by screening Phetchabun residents ages 35 to 69 years for anti-HCV antibodies by using a rapid diagnostic test (RDT) at the point of care. Positive anti-HCV results were further evaluated for active infection using qualitative HCV RNA assay, followed by quantitative HCV viral load determination in a subset of samples. Currently, we have identified 6.2% (10,621/170,163) anti-HCV positive individuals, of whom 74.9% (3,930/5,246) demonstrated detectable viral RNA. Quantitative test found that 97.5% (1,001/1,027) had HCV viral load ≥5,000 IU/mL. Thus, primary screening with anti-HCV RDT followed by qualitative HCV RNA evaluation could identify active and chronic HCV infection in almost all individuals with a viral load ≥5,000 IU/mL, which is the current threshold for treatment dictated by Thailand’s National Health Security Office. Our data suggest that qualitative HCV RNA evaluation may obviate the need for the more expensive quantitative HCV viral load test and reduce a significant barrier toward HCV elimination in a middle-income country.

Funder

National Research Council, Thailand Grand Challenge Fund

National Research Council; Grant for New Researcher

Center of Excellence in Clinical Virology at Chulalongkorn University and Hospital

The Second Century Fund of Chulalongkorn University for Postdoctoral Fellowship

Publisher

Public Library of Science (PLoS)

Subject

Multidisciplinary

Reference21 articles.

1. Hepatitis C;CW Spearman;Lancet,2019

2. Epidemiology of hepatitis C virus infection;F. Roudot-Thoraval;Clin Res Hepatol Gastroenterol,2021

3. Organization WH. Hepatitis C 2021 [cited 2021 August 25]. Available from: https://www.who.int/news-room/fact-sheets/detail/hepatitis-c.

4. Diagnosis and management of hepatitis C;LJ Vine;Br J Hosp Med (Lond),2015

5. Epidemiology of hepatitis C: geographic differences and temporal trends;A Wasley;Semin Liver Dis,2000

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