Abstract
Deriving new value from waste streams through secondary processes is a central aim of the circular bioeconomy. In this study we investigate whether chemically defined spent media (CDSM) waste from cell culture bioprocess can be recycled and used as a feed in secondary microbial fermentation to produce new recombinant protein products. Our results show that CDSM supplemented with 2% glycerol supported a specific growth rate of E. coli cultures equivalent to that achieved using a nutritionally rich microbiological media (LB). The titre of recombinant protein produced following induction in a 4-hour expression screen was approximately equivalent in the CDSM fed cultures to that of baseline, and this was maintained in a 16-hr preparative fermentation. To understand the protein production achieved in CDSM fed culture we performed a quantitative analysis of proteome changes in the E. coli using mass spectrometry. This analysis revealed significant upregulation of protein synthesis machinery enzymes and significant downregulation of carbohydrate metabolism enzymes. We conclude that spent cell culture media, which represents 100s of millions of litres of waste generated by the bioprocessing industry annually, may be valorized as a feed resource for the production of recombinant proteins in secondary microbial fermentations. Data is available via ProteomeXchange with identifier PXD026884.
Funder
Science Foundation Ireland
Engineering and Physical Sciences Research Council
Publisher
Public Library of Science (PLoS)
Reference43 articles.
1. The circular bioeconomy: Its elements and role in European bioeconomy clusters;P Stegmann;Resources, Conservation & Recycling: X,2020
2. Mammalian Biomanufacturing Industry Supply & Demand Trends [Internet];Ecker and Patricia Seymour D;BioProcess Online,2020
3. Cell culture media for recombinant protein expression in Chinese hamster ovary (CHO) cells: History, key components, and optimization strategies;FV Ritacco;Biotechnol Prog,2018
4. Advances in recombinant antibody manufacturing;R Kunert;Appl Microbiol Biotechnol,2016
5. Re-use of spent cell culture medium in pilot scale and rapid preparative purification with membrane chromatography;U Riese;J Biotechnol,1994
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献