mmu-miRNA-342-3p promotes hepatic stellate cell activation and hepatic fibrosis induced by Echinococcus multilocularis infection via targeting Zbtb7a
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Published:2023-07-25
Issue:7
Volume:17
Page:e0011520
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ISSN:1935-2735
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Container-title:PLOS Neglected Tropical Diseases
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language:en
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Short-container-title:PLoS Negl Trop Dis
Author:
Cao Shanling,
Wang Dexian,
Wu Yixuan,
Zhang Junmei,
Pu Lixia,
Luo Xuenong,
Zhang Xueyong,
Sun Xiaolin,
Zheng Yadong,
Wang Shuai,
Guo XiaolaORCID
Abstract
Liver fibrosis is one of the histopathological characters duringEchinococcus multilocularisinfection. The activation of hepatic stellate cells (HSCs) is a key event in the development of liver fibrosis. However, the molecular mechanism of HSC activation in theE.multilocularisinfection-induced liver fibrosis remains largely unclear. Here, we reported that mmu-miR-342-3p was most dominantly expressed in HSCs and was upregulated in the HSCs in response toE.multilocularisinfection. We further showed that mmu-miR-342-3p was able to bind to the 3’ UTR of theZbtb7agene and regulated its expression. Moreover, mmu-miR-342-3p expression was negatively correlated with its target geneZbtb7ain HSCs duringE.multilocularisinfection. Knockdown of mmu-miR-342-3p promoted the expression ofGfapin the activated HSCsin vitro. In theE.multilocularis-infected mice, knockdown of mmu-miR-342-3p suppressed the expression ofα-Sma,Col1α1, andTGF-βbut promoted the expression ofGfap. Therefore, mmu-miR-342-3p is a key regulator for activation of HSCs, and inhibiting mmu-miR-342-3p to suppressed Zbtb7a-mediated TGF-β signaling in activated HSCs could be a novel strategy to treat liver fibrosis induced byE.multilocularis.
Funder
National Key Research and Development Program of China
National Natural Science Foundation of China
Tarimsal Araştirmalar ve Politikalar Genel Müdürlüğü, Türkiye Cumhuriyeti Tarim Ve Orman Bakanliği
State Key Laboratory of Drug Research
Publisher
Public Library of Science (PLoS)
Subject
Infectious Diseases,Public Health, Environmental and Occupational Health