Elevated ozone and carbon dioxide affects the composition of volatile organic compounds emitted by Vicia faba (L.) and visitation by European orchard bee (Osmia cornuta)

Abstract

Recent studies link increased ozone (O3) and carbon dioxide (CO2) levels to alteration of plant performance and plant-herbivore interactions, but their interactive effects on plant-pollinator interactions are little understood. Extra floral nectaries (EFNs) are essential organs used by some plants for stimulating defense against herbivory and for the attraction of insect pollinators, e.g., bees. The factors driving the interactions between bees and plants regarding the visitation of bees to EFNs are poorly understood, especially in the face of global change driven by greenhouse gases. Here, we experimentally tested whether elevated levels of O3and CO2individually and interactively alter the emission of Volatile Organic Compound (VOC) profiles in the field bean plant (Vicia faba, L., Fabaceae), EFN nectar production and EFN visitation by the European orchard bee (Osmia cornuta, Latreille, Megachilidae). Our results showed that O3alone had significant negative effects on the blends of VOCs emitted while the treatment with elevated CO2alone did not differ from the control. Furthermore, as with O3alone, the mixture of O3and CO2also had a significant difference in the VOCs’ profile. O3exposure was also linked to reduced nectar volume and had a negative impact on EFN visitation by bees. Increased CO2level, on the other hand, had a positive impact on bee visits. Our results add to the knowledge of the interactive effects of O3and CO2on plant volatiles emitted byVicia fabaand bee responses. As greenhouse gas levels continue to rise globally, it is important to take these findings into consideration to better prepare for changes in plant-insect interactions.