Concentration and detection of SARS coronavirus in sewage from Xiao Tang Shan hospital and the 309th Hospital of the Chinese People's Liberation Army

Author:

Wang X.W.1,Li J.2,Guo T.3,Zhen B.2,Kong Q.1,Yi B.4,Li Z.1,Song N.1,Jin M.1,Xiao W.2,zhu X.3,Gu C.1,Yin J.1,Wei W.2,Yao W.3,Liu C.5,Li J.3,Ou G.1,Wang M.2,Fang T.6,Wang G.2,Qiu Y.3,Wu H.3,Chao F.1,Li J.1

Affiliation:

1. Institute of Hygiene and Environmental Medicine, Academy of Military Medical Sciences, 1 Da Li Road, Tianjin, 300050, P.R. China

2. Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, 172 Dong Da Jie street, Feng Tai, Beijing, 100072, P.R. China

3. Xiao Tang Shang Hospital, Beijing, 102211, P.R. China

4. 309 Hospital of PLA of China, 17 Mushanhu Road, Beijing, 100091, P.R. China

5. Beijing Institute of Pharmacology & Toxicology, 100850, P.R. China

6. Beijing Institute of Basic Medicine, 100850, P.R. China

Abstract

A worldwide outbreak of severe acute respiratory syndrome (SARS) had been reported. Over 8439 SARS cases and 812 SARS-related deaths were reported to the World Health Organization from 32 countries around the world up to 5 July 2003. The mechanism of transmission of SARS-CoV has been limited only to close contacts with patients. Attention was focused on possible transmission by the sewage system because laboratory studies showed that patients excreted coronavirus RNA in their stools in Amoy Gardens in Hong Kong. To explore whether the stool of SARS patients or the sewage containing the stool of patients would transmit SARS-CoV or not, we used a style of electropositive filter media particle to concentrate the SARS-CoV from the sewage of two hospitals receiving SARS patients in Beijing, as well as cell culture, semi-nested RT–PCR and sequencing of genes to detect and identify the viruses from sewage. There was no live SARS-CoV detected in the sewage in these assays. The nucleic acid of SARS-CoV was found in the sewage before disinfection from both hospitals by PCR. After disinfection, SARS-CoV RNA could be detected from some samples from the 309th Hospital of the Chinese People's Liberation Army, but not from Xiao Tang Shan Hospital after disinfection. In this study, we found that the virus can survive for 14 days in sewage at 4°C, 2 days at 20°C, and its RNA can be detected for 8 days though the virus had been inactivated. In conclusion, this study demonstrates that the RNA of SARS-CoV could be detected from the concentrates of sewage of both hospitals receiving SARS patients before disinfection and occasionally after disinfection though there was no live SARS-CoV; thus much attention should be paid to the treatment of stools of patients and the sewage of hospitals receiving SARS patients.

Publisher

IWA Publishing

Subject

Water Science and Technology,Environmental Engineering

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3