Affiliation:
1. Cotton Genetics Laboratory, National University of Uzbekistan, Tashkent, Vuzgorodok 100174, Uzbekistan
2. Center of Genomic Technologies, Institute of Genetics and Plant Experimental Biology, Academy of Sciences of Uzbekistan, Yuqori Yuz, Qibray Region, Tashkent 111226, Uzbekistan
Abstract
The use of aneuploid lines significantly increases the effectiveness of molecular-genetic analysis and the development of superior quality breeding lines via substitutions by alien chromosomes. To date, however, a complete set of aneuploid series for each cotton chromosome is not available. Here, we present the development of a monosomic stock collection of cotton (Gossypium hirsutumL.) from Uzbekistan, including the origin of 92 primary monosomics, meiotic metaphase-I analysis, study of tetrads of microspores, pollen fertility, and monosomic transmission rates for some monosomic lines. We report desynaptic effects of some monosomes detected both in parental and daughter monosomics, a positive role of interchanges in translocation heterozygous monosomics due to selective advantages of gametes with deficiency and a simultaneous interchange, pollen fertility variation, and strong differences in transmission rates. This monosomic cotton collection, developed using single genome background, will be useful for future breeding, genetic, cytogenetic, and molecular-genetic investigations of the cotton genome.
Funder
Committee for Science and Technology of the Republic of Uzbekistan
Subject
Genetics (clinical),Genetics,Molecular Biology
Cited by
4 articles.
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