An epidemic CC1-MRSA-IV clone yields false-negative test results in molecular MRSA identification assays: a note of caution, Austria, Germany, Ireland, 2020

Author:

Monecke Stefan1234,König Elisabeth51,Earls Megan R61,Leitner Eva5ORCID,Müller Elke24,Wagner Gabriel E5,Poitz David M7,Jatzwauk Lutz8,Vremerǎ Teodora9,Dorneanu Olivia S9,Simbeck Alexandra10,Ambrosch Andreas11,Zollner-Schwetz Ines12,Krause Robert12,Ruppitsch Werner13,Schneider-Brachert Wulf10,Coleman David C6,Steinmetz Ivo5,Ehricht Ralf1424

Affiliation:

1. These authors contributed equally

2. InfectoGnostics Research Campus Jena, Jena, Germany

3. Institute for Medical Microbiology and Hygiene, Medical Faculty ‘Carl Gustav Carus’, Technische Universität Dresden, Dresden, Germany

4. Leibniz Institute of Photonic Technology (IPHT), Jena, Germany

5. Diagnostic and Research Institute for Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, Graz, Austria

6. Microbiology Research Unit, Division of Oral Biosciences, Dublin Dental University Hospital, Trinity College, University of Dublin, Dublin, Ireland

7. Institute of Clinical Chemistry and Laboratory Medicine, University Hospital ‘Carl Gustav Carus’, Technische Universität Dresden, Dresden, Germany

8. Department of Hospital Infection Control, University Hospital ‘Carl Gustav Carus’, Technische Universität Dresden, Dresden, Germany

9. Microbiology Unit, Department of Preventive and Interdisciplinary Medicine, University of Medicine and Pharmacy ‘Grigore T Popa’, Iaşi, Romania

10. Department of Infection Control and Infectious Diseases, University Hospital Regensburg, Regensburg, Germany

11. Institute of Laboratory Medicine, Microbiology and Hygiene, Barmherzige Brüder Hospital, Regensburg, Germany

12. Section of Infectious Diseases and Tropical Medicine, Department of Internal Medicine, Medical University of Graz, Austria

13. Institute of Medical Microbiology and Hygiene, Austrian Agency for Health and Food Safety, Vienna, Austria

14. Friedrich- Schiller University; Institute of Physical Chemistry, Jena, Germany

Abstract

We investigated why a clinical meticillin-resistant Staphylococcus aureus (MRSA) isolate yielded false-negative results with some commercial PCR tests for MRSA detection. We found that an epidemic European CC1-MRSA-IV clone generally exhibits this behaviour. The failure of the assays was attributable to a large insertion in the orfX/SCCmec integration site. To ensure the reliability of molecular MRSA tests, it is vital to monitor emergence of new SCCmec types and junction sites.

Publisher

European Centre for Disease Control and Prevention (ECDC)

Subject

Virology,Public Health, Environmental and Occupational Health,Epidemiology

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