External quality assessment of orthohantavirus and lymphocytic choriomeningitis virus molecular detection and serology in Europe, 2021

Author:

Erdin Mert12ORCID,Stanoeva Kamelia R13ORCID,Mögling Ramona3ORCID,Korva Miša4ORCID,Knap Nataša4ORCID,Resman Rus Katarina4ORCID,Domingo Cristina5ORCID,Reimerink Johan HJ3ORCID,de Vries Ankje3ORCID,Alburkat Hussein2ORCID,Utriainen Mira2ORCID,Gossner Céline M6ORCID,Sironen Tarja72ORCID,Avšič-Županc Tatjana84ORCID,Reusken Chantal BEM83ORCID,Vapalahti Olli7829ORCID

Affiliation:

1. These authors contributed equally to the work and share the first authorship.

2. Department of Virology, Medicum, Faculty of Medicine, University of Helsinki, Helsinki, Finland

3. Centre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands

4. Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia

5. Centre for Biological Threats and Special Pathogens, Robert Koch Institute (RKI), Berlin, Germany. Current affiliation: Centre for International Health Protection, RKI, Berlin, Germany

6. Diseases Programme Unit, European Centre for Disease Prevention and Control (ECDC), Solna, Sweden

7. Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland

8. These authors contributed equally to the work and share the last authorship.

9. Helsinki University Hospital Diagnostic Center, HUSLAB, Helsinki, Finland

Abstract

Background Rodent-borne viruses such as orthohantaviruses and arenaviruses cause considerable disease burden with regional and temporal differences in incidence and clinical awareness. Therefore, it is important to regularly evaluate laboratory diagnostic capabilities, e.g. by external quality assessments (EQA). Aim We wished to evaluate the performance and diagnostic capability of European expert laboratories to detect orthohantaviruses and lymphocytic choriomeningitis virus (LCMV) and human antibody response towards orthohantaviruses. Methods We conducted an EQA in 2021; molecular panels consisted of 12 samples, including different orthohantaviruses (Seoul, Dobrava-Belgrade (DOBV), Puumala (PUUV) and Hantaan orthohantavirus), LCMV and negative controls. Serological panels consisted of six human serum samples reactive to PUUV, DOBV or negative to orthohantaviruses. The EQA was sent to 25 laboratories in 20 countries. Results The accuracy of molecular detection of orthohantaviruses varied (50‒67%, average 62%) among 16 participating laboratories, while LCMV samples were successfully detected in all 11 participating laboratories (91-100%, average 96%). The accuracy of serological diagnosis of acute and past orthohantavirus infections was on average 95% among 20 participating laboratories and 82% in 19 laboratories, respectively. A variety of methods was used, with predominance of in-house assays for molecular tests, and commercial assays for serological ones. Conclusion Serology, the most common tool to diagnose acute orthohantavirus infections, had a high accuracy in this EQA. The molecular detection of orthohantaviruses needs improvement while LCMV detection (performed in fewer laboratories) had 95% accuracy. Further EQAs are recommended to be performed periodically to monitor improvements and challenges in the diagnostics of rodent–borne diseases.

Publisher

European Centre for Disease Control and Prevention (ECDC)

Subject

Virology,Public Health, Environmental and Occupational Health,Epidemiology

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