Incorporation of oxygen-18 into the 25-position of cholecalciferol by hepatic cholecalciferol 25-hydroxylase

Author:

Madhok T C,Schnoes H K,DeLuca H F

Abstract

The oxygen enzymically inserted as a hydroxy function by rat liver post-mitochondrial fraction into the 25-position of cholecalciferol to giver 25-hydroxycholecaliferol is derived exclusively from molecular O2. Therefore like the other two cholecalciferol hydroxylases, i.e. 25-hydroxycholecalciferol 1alpha-hydroxylase and 25-hydroxycholecalciferol 24-hydroxylase, the cholecalciferol 25-hydroxylase is also a mono-oxygenase (‘mixed-function oxidase’).

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry

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