The complexity of satellite deoxyribonucleic acid in a higher plant-Reference-Cited by-同舟云学术

The complexity of satellite deoxyribonucleic acid in a higher plant

Published:1975-07-01 Issue:1 Volume:149 Page:31-38
ISSN:0264-6021
Container-title:Biochemical Journal
language:en
Short-container-title:

Author:

Sinclair J¹,Wells R¹,Deumling B¹,Ingle J¹

Affiliation:

1. Department of Botany, University of Edinburgh, Edinburgh EH9 3JH, U.K.

Abstract

Purified satellite DNA from melon (Cucumis melo) was shown to contain at least two components from thermal-denaturation and renaturation studies. Two components were separated after partial renaturation, a fast-renaturing fraction similar in complexity to mouse satellite DNA, and one with 6000 times greater complexity. Both components renatured very accurately, indicating a minimum of sequence divergence. Centrifugation of the purified satellite DNA in Ag+/Cs2SO4 gradients resolved two major and several minor fractions. The two major fractions were only slightly enriched for fast- or slow-renaturing sequences.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry

Link

https://portlandpress.com/biochemj/article-pdf/149/1/31/565082/bj1490031.pdf

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1. Analysis and in situ hybridization of cryptic satellites in Hordeum arizonicum;Theoretical and Applied Genetics;1986-11

2. Characterization and localization of cryptic satellite DNAs in barley (Hordeum vulgare);Theoretical and Applied Genetics;1986-11

3. Isopycnic Centrifugation of DNA;Springer Protocols Handbooks;1986

4. Characterisation of GC-rich Satellite DNA from Sinapis alba;Zeitschrift für Pflanzenphysiologie;1982-12

5. Organization of highly repetitive satellite DNA of two Cucurbitaceae species (Cucumis melo and Cucumis sativus);Nucleic Acids Research;1982