Affiliation:
1. Department of Biochemistry, University of Sheffield, Sheffield S10 2TN, U.K.
Abstract
1. Two oxaloacetate decarboxylases of rat liver are described; one is mitochondrial with no metal ion requirement and activity in the alkaline pH region; the other is cytoplasmic with Mn2+or Mg2+requirement and activity around pH5. 2. A method for the partial purification of the mitochondrial enzyme is described. 3. The apparent Km of the mitochondrial enzyme is 0.23mm. 4. Inhibition of the mitochondrial enzyme by substrate, CoA, acetyl-CoA, citrate and phosphoenolpyruvate is described.
Subject
Cell Biology,Molecular Biology,Biochemistry
Cited by
22 articles.
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