Affiliation:
1. Department of Clinical Laboratory Medicine, The University of Tokyo, Tokyo, Japan
Abstract
Abstract
Background: Liver regeneration is an extremely complicated process that is regulated by a number of signaling pathways. Sphingosine 1-phosphate (S1P), a potent bioactive lipid mediator playing crucial roles in various cellular responses through its receptors, has been attracting attention in the fields of hepatology, where S1P lyase (SPL), an irreversibly degrading enzyme of S1P, reportedly has a stimulatory role in growth of hepatocellular carcinoma (HCC).
Aim of the study: To examine whether SPL might play a stimulatory role in liver regeneration.
Method: Using in-vivo siRNA technology, we inhibited SPL expression. Seventy percent of the liver was resected in mice as partial hepatectomy (PH). Liver tissue samples were collected and mRNA expression level of the SPL, IHC of the proliferating cell nuclear antigen (PCNA), protein levels of various proliferation factors and lipid measurements were performed in different groups.
Results: The mRNA levels of SPL increased in PH mice on the third day after PH surgery. When we suppressed the expression of SPL by in-vivo siRNA, we observed a significant decline of the PCNA positive cell numbers. Furthermore, the Cyclin D1 expressions and phosphorylation of ERK also were decreased in the siSPL injected PH group.
Conclusion: We verified the importance of the SPL in liver regeneration, using the mice PH model. SPL might be a potential target to facilitate liver regeneration.
Subject
Cell Biology,Molecular Biology,Biochemistry,Biophysics
Reference37 articles.
1. A morphological study of regeneration of the liver after partial removal;Fishback;Arch. Pathol.,1929
2. Principles of liver regeneration and growth homeostasis;Michalopoulos;Compr. Physiol.,2013
3. Regeneration of rat liver: transfer of humoral agent by cross circulation;Moolten;Science,1967
4. Understanding the marvels behind liver regeneration;Abu Rmilah;Wiley Interdiscip. Rev. Dev. Biol.,2019
5. Liver regeneration;Fausto;Hepatology,2006
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献