In vitro translation in a hybrid cell free lysate with exogenous cellular ribosomes-Reference-Cited by-同舟云学术

In vitro translation in a hybrid cell free lysate with exogenous cellular ribosomes

Published:2015-04-17 Issue:3 Volume:467 Page:387-398
ISSN:0264-6021
Container-title:Biochemical Journal
language:en
Short-container-title:

Author:

Panthu Baptiste¹²³⁴⁵,Décimo Didier¹²³⁴⁵,Balvay Laurent¹²³⁴⁵,Ohlmann Théophile¹²³⁴⁵

Affiliation:

1. CIRI, International Center for Infectiology Research, Université de Lyon, Lyon, France

2. Inserm, Virology Unit 1111, Lyon, France

3. Ecole Normale Supérieure de Lyon, Lyon, France

4. Université Lyon 1, Centre International de Recherche en Infectiologie, Lyon, France

5. CNRS, Unitémixte de Recherche 5308, Lyon, France

Abstract

This describes a novel cell-free system in which endogenous ribosomes from the reticulocyte lysate are replaced by ribosomes from any tissues, organs or cell types. This enables to control the overall ribosomal concentration and to study the translation of specialized genes.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry

Link

https://portlandpress.com/biochemj/article-pdf/467/3/387/681836/bj4670387.pdf

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