Affiliation:
1. Department of Botany, University College, Swansea SA2 8PP, U.K.
Abstract
The synthesis of the pyrimidinyl amino acids willardiine and isowillardiine was studied in vivo and in vitro. Uracil derivatives stimulate the biosynthesis of both compounds; the free base is the most effective. Significant incorporation of [2-14C]uracil and [6-14C]orotate into willardiine and isowillardiine was found. Incorporation of [6-14C]orotate was substantially decreased in the presence of uracil, and to a lesser extent by uridine and UMP. [3-14C]Serine was incorporated into the alanine side chain of the two uracilylalanines but not into the ring. The effect of a number of uracil analogues and inhibitors of pyrimidine metabolism was examined. Some were shown to stimulate the biosynthesis; the most noticeable effects were obtained with 6-azauracil and 2-thiouracil. Attempts to obtain extracts capable of synthesizing the uracilylalanines from uracil and serine were unsuccessful, but weak activity was observed when serine was replaced by O-acetylserine.
Cited by
25 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
1. Biochemistry of plants N–heterocyclic non-protein amino acids;Amino Acids;2021-05-05
2. Uridine Function in the Central Nervous System;Current Topics in Medicinal Chemistry;2011-04-01
3. Polymers Containing the 1,2,4-Triazine Nucleus;Chemistry of Heterocyclic Compounds: A Series Of Monographs;2008-01-02
4. Non-Protein Amino Acids: A Review of the Biosynthesis and Taxonomic Significance;Natural Product Communications;2008-01
5. The Nonprotein Amino Acids of Plants;Advances in Enzymology - and Related Areas of Molecular Biology;2006-11-22