Koyun Alveoler Makrofajlarında Mannheimia Haemolytica'ya Yanıt Olarak Bağışıklık ile İlişkili Genlerin İn Vitro mRNA Ekspresyon Dinamikleri

Author:

AL-JANABI Saif Adil Abbood1ORCID,ÇINAR Mehmet Ulaş2ORCID,SAJİD Ghulam Asghar1ORCID,NAJİ Ahmed Qasim Naji1ORCID,SOHEL Md Mahmodul Hasan2ORCID

Affiliation:

1. ERCİYES ÜNİVERSİTESİ

2. ERCIYES UNIVERSITY

Abstract

Alveolar macrophages (AMs) respond to these infections as the first line of defense and trigger the lung's immune response. Knowing the expression dynamics of key immune-related genes in alveolar macrophages against M. haemolytica challenge will help deepen the understanding of disease immunopathogenesis. For this purpose, first time an in vitro obtained from bronchoalveolar lavage (BAL) fluid and treated with three doses (T1: 1800, T2: 2700, T3: 5400 CFU) of M. haemolytica inoculum, while keeping one untreated as a control. Then, total RNA was extracted, and cDNA was synthesized. The real-time quantification data indicated that the expression of IL-1β, IL-6, IL-8, IL-10, and NF-κB genes were significantly changed as compared to control. Our study revealed that the exposure to M. haemolytica stimulates the immune response in the sheep alveolar macrophages in a dose and time-dependent manner.

Funder

Erciyes University Scientific Research Projects Unit

Publisher

Turkish Federation of Animal Science (Zootekni Federasyonu)

Subject

General Engineering

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