The appearance and activity of tissue thyroxine 5′- and 5-monodeiodinase during ontogenesis in the fetal pig

Abstract

Abstract The ontogeny of the fetal monodeiodinase systems, during the period before the onset of thyroid activity until birth, has been described only in the rat, rabbit and chick. We have studied 5′- and 5-monodeiodinase activities in the liver, kidney and placenta of pig fetuses, from day 32 of gestation up to birth, and on days 1, 3, 7 and 14 after birth. Fetuses (123) from 15 litters, 32 newborn piglets, 15 sows (mothers) and 10 non-pregnant pigs were used. The relationships between monodeiodinase activities, thyroxine (T4), tri-iodothyronine (T3) and reverse triiodothyronine (rT3) blood serum concentrations and sulphydryl groups (total (T-SH) and non-protein (NP-SH)), were measured. 5′-Monodeiodinase activity (5′-MD type I; converting T4 to T3) and 5-monodeiodinase (5-MD; converting T4 to rT3) were detectable in the liver at the onset of thyroid hormone biosynthesis, and before that time (day 32 of gestation) in the placenta. Liver 5′-MD activity was very low between days 32 and 84 of gestation, and then increased rapidly at about day 93, reaching a maximal value 2 days before term. Activity in the liver (but not in the kidney) was higher than that in maternal, newborn and non-pregnant adult tissue. The increase in NP-SH groups in the liver and kidney and T-SH groups in the placenta during the last 5 days of gestation coincided with the increase in 5′-MD activity. The 5-MD activity of the liver was generally five to ten times lower than that of 5′-MD, being highest on days 65–76 and 65–84 in the kidney and placenta respectively. Serum T4 concentrations paralleled the steady increase in T4- to T3-converting activity in the kidney and liver, but not in the placenta. Two weeks before birth, fetal T3 concentrations exceeded those of rT3 by a factor of 1·5 or more, indicating that T4 metabolism in the pig is dominated by the activity of 5′-MD. This pattern is different from that in prenatal humans, rats or sheep in which rT3 is the primary circulating T4 metabolite. Journal of Endocrinology (1994) 141, 243–249