Primary cilia sensitize endothelial cells to BMP and prevent excessive vascular regression

Author:

Vion Anne-Clémence123ORCID,Alt Silvanus1,Klaus-Bergmann Alexandra13ORCID,Szymborska Anna13ORCID,Zheng Tuyu1ORCID,Perovic Tijana1ORCID,Hammoutene Adel45,Oliveira Marta Bastos1ORCID,Bartels-Klein Eireen13,Hollfinger Irene1,Rautou Pierre-Emmanuel456,Bernabeu Miguel O.78ORCID,Gerhardt Holger123910ORCID

Affiliation:

1. Max Delbrück Center for Molecular Medicine, Berlin, Germany

2. Vascular Biology Laboratory, London Research Institute – Cancer Research UK, Lincoln’s Inn Fields Laboratories, London, England, UK

3. German Center for Cardiovascular Research, Berlin, Germany

4. Institut National de la Santé et de la Recherche Medicale, U970, Paris Cardiovascular Research Center, Paris, France

5. Université Paris Descartes, Sorbonne Paris Cité, Paris, France

6. Departement Hospitalo-Universitaire Unity, Pôle des Maladies de l’Appareil Digestif, Service d'Hépatologie, Centre de Référence des Maladies Vasculaires du Foie, Hôpital Beaujon, Assistance Publique - Hopitaux de Paris, Clichy, France

7. Centre for Medical Informatics, Usher Institute, University of Edinburgh, Edinburgh, Scotland, UK

8. Centre for Computational Science, Department of Chemistry, University College London, London, England, UK

9. Vascular Patterning Laboratory, VIB Center for Cancer Biology, Leuven, Belgium

10. Vascular Patterning Laboratory, Department of Oncology, KU Leuven, Leuven, Belgium

Abstract

Blood flow shapes vascular networks by orchestrating endothelial cell behavior and function. How endothelial cells read and interpret flow-derived signals is poorly understood. Here, we show that endothelial cells in the developing mouse retina form and use luminal primary cilia to stabilize vessel connections selectively in parts of the remodeling vascular plexus experiencing low and intermediate shear stress. Inducible genetic deletion of the essential cilia component intraflagellar transport protein 88 (IFT88) in endothelial cells caused premature and random vessel regression without affecting proliferation, cell cycle progression, or apoptosis. IFT88 mutant cells lacking primary cilia displayed reduced polarization against blood flow, selectively at low and intermediate flow levels, and have a stronger migratory behavior. Molecularly, we identify that primary cilia endow endothelial cells with strongly enhanced sensitivity to bone morphogenic protein 9 (BMP9), selectively under low flow. We propose that BMP9 signaling cooperates with the primary cilia at low flow to keep immature vessels open before high shear stress–mediated remodeling.

Funder

Britain Israel Research and Academic Exchange Partnership

German Centre for Cardiovascular Research

German Ministry of Education and Research

European Research Council

European Molecular Biology Organization

Agence Nationale pour la Recherche

Engineering and Physical Sciences Research Council

ARCHER UK National Supercomputing Service

Publisher

Rockefeller University Press

Subject

Cell Biology

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3