Staining of Tissue Sections for Electron Microscopy with Heavy Metals

Author:

Watson Michael L.1

Affiliation:

1. (From the Departments of Pathology and Radiation Biology, University of Rochester School of Medicine and Dentistry, Rochester, New York)

Abstract

Descriptions of three heavy metal stains and methods of application to tissue sections for electron microscopy are presented. Lead hydroxide stains rather selectively two types of particles in liver: those associated with the endoplasmic reticulum and containing ribonucleic acid and other somewhat larger particles. Barium hydroxide emphasizes certain bodies within vesicles of the Golgi region of hepatic cells. Alkalized lead acetate is useful as a general stain, as are also lead and barium hydroxides.

Publisher

Rockefeller University Press

Subject

Cell Biology

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