Principle Development for Milk Enrichment with Nano-Sized Forms of the Essential Trace Element Selenium

Abstract

Selenium plays an important role in the human body; its deficiency causes endemic cardiomyopathy, carcinogenic processes acceleration in the prostate gland, and fertility disfunction. The study aimed at the principle development of milk enrichment with nanoscale forms of the essential trace element selenium. A man synthesized selenium nanoparticle, stabilized with Kolliphor HS15 by chemical reduction in an aqueous medium; ascorbic acid acted as a reducer. The researchers conducted multifactorial experiment to study the selenium nanoparticle stability at various technological parameter values; obtained distribution histograms of the hydrodynamic radius; determined the size of the selenium particles by the dynamic light scattering method. Furthermore, the authors studied the technological parameters impact on the resulting nanoscale system stability. As a result of neural network processing of experimental data, a man formed ternary surface; and determined the optimal parameters for obtaining selenium nanoparticles with the smallest radius based on the surface analysis. In the experiment, the researchers used full– cream unpasteurized milk with a fat content of 3.2%. They run enrichment at the rate of 19 micrograms per 100 ml of milk, which corresponds to 30% of the daily selenium value. Subsequently, a man pasteurized milk samples enriched with selenium nanoparticles at 72 °C for 2 minutes and measured physico-chemical parameters. As a result, the antioxidant activity of milk samples remained constant regardless of the stage of enrichment with a selenium– containing additive. A man can conduct the enrichment with a selenium-containing additive both before and after milk pasteurization.