Effect of Iron Overload on Tight Junctions and Adhesion Molecules in ECV304 Cells

Author:

Sar Samed Refik,Meric Furkan,Yanik Ilgar Aysegul,Albayrak Irem Gulfem,Arslan Belkis Atasever

Abstract

Blood vessels are essential for the body’s tissues and organs to receive oxygen and nutrition. The Blood-Brain Barrier (BBB) is a special feature of the blood arteries that vascularise the Central Nervous System (CNS) which enables these vessels to tightly control the flow of ions and molecules between the blood and the brain. The accurate regulation of CNS homeostasis facilitates appropriate neuronal performance and safeguards neural tissue from toxins and pathogens. Modifications to these barrier characteristics play a significant role in the development of many neurological disorders. BBB contains tight junction transmembrane proteins, integral membrane proteins, occludin, claudins, IgG-type proteins, junction adhesion molecules and scaffold proteins. Numerous biological functions require iron in the CNS, including neurotransmitter synthesis, myelin formation and mitochondrial function. However, excess iron can lead to oxidative stress and damage, which are implicated in neurodegenerative diseases. In this research, the impacts of iron accumulation on Cadherin 5 (CDH5), Claudin 5 (CLDN5), Intercellular Adhesion Molecule 1 (ICAM-1), Occludin (OCL), p-selectin (P-SEL), Vascular Cell Adhesion Molecule 1 (VCAM-1) and Zonula occludens-1 (ZO-1) genes expressions in Human Umbilical Vein Endothelial Cells (ECV 304) cells were investigated. It was found that in human umbilical vein endothelial cell line cells, iron overload enhanced the expression of CDH5 and P-SEL genes while reducing the expression of VCAM1, Cldn5, ICAM-1, OCL, and Zo-1 genes.

Publisher

Informatics Publishing Limited

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