STRUCTURE ELUCIDATION OF BIOACTIVE MATERIAL FROM STREPTOMYCES SPORORAVEUS AND ITS BIOLOGICAL ACTIVITY (PATHOGENIC MICROORGANISMS AND CYTOTOXICITY)

Abstract

Filamentous bacteria belonging to the Streptomyces genus are well known for their exceptional capacity to generate a diverse range of bioavailable secondary metabolites. In this work, an inoculum for fermentation was created using Streptomyces sp., which was isolated from Yanbu City, Saudi Arabia. It was 99% identical to Streptomyces spororaveus AD 4, according to 16S ribosomal RNA sequence homology studies. After using ethyl acetate to extract the fermented broth, the crude extract's purity was checked using high-performance liquid chromatography (HPLC). Following the purification stages, three pure bioactive fractions were isolated and given the names A, B, and C, with retention times of 2.251 ± 0.3 min, 4.342 ± 0.3 min, and 5.988 ± 0.3 min, respectively. Additionally, the spectroscopic examinations of these three compounds, including their GC-MS data, were used to determine their chemical structures by comparing them to published reference data. Additionally, the three fractions exhibit potent antimicrobial activity against Salmonella typhi ATCC 14028, Candida albicanc, Aspergillus niger, Klebsiella pneumoniae, Pseudomonas aeruginosa ATCC 9027, Staphylococcus epidermidis, Micrococcus luteus, E. coli ATCC 8739, and B. subtilis ATCC 6633. Also, the cytotoxicity of the bioactive fractions was assessed using the Hct-116 line. Of the three fractions, fraction C had the lowest cytotoxicity, with an IC50 value of 224.57 ± 5.82, followed by fraction B at 238.82 ± 2.57 and fraction A at 341.28 ± 1.69.