Research on the stability changes in expert consensus of the ACTH detection preprocessing scheme
Author:
Dong Lizhen1, Hao Tianqi1, Chen Jiawei1, Chai Yamin1, Jia Zichao1, Tuo Wenbin2, Yuan Chunhui2, Luo Wei1ORCID
Affiliation:
1. Department of Clinical Laboratory , General Hospital of Tianjin Medical University , Tianjin , P.R. China 2. Department of Laboratory Medicine , Wuhan Children’s Hospital (Wuhan Maternal and Child Health Care Hospital), Tongji Medical College, Huazhong University of Science & Technology , Wuhan , P.R. China
Abstract
Abstract
Objectives
Adrenocorticotropic hormone (ACTH) is extremely unstable and can easily degrade at room temperature. The experts agreed that the samples should be transported in an ice bath. If it cannot be detected immediately, the plasma should be separated and frozen, which is difficult to carry out in routine practice. This study was performed to explore the preanalytical factors that influence the stability of adrenocorticotrophic hormone (ACTH) measurements.
Methods
ACTH levels in 21 EDTA whole-blood samples were measured immediately after 0 h and then divided into three equal groups according to the corresponding values (low, L; median, M; and high, H). Next, three sample processing methods (including seven subtypes) were used: whole blood was uncentrifuged (named the A method), stored at 4 °C or 22 °C, centrifuged but not subjected to plasma removal (the B method), stored at 4 °C or 22 °C, and centrifuged with the plasma removed and stored (the C method) at 4 °C, 22 °C, and −20 °C. Each subtype contained three samples, namely, L, M, and H; these samples were retested using a Siemens XP2000 at different times. The change bias was calculated at 0 h.
Results
Compared to that at 0 h, there was no significant change in ACTH up to 24 h when the sample was stored at 4 °C or 22 °C with the B method (p>0.05), while it significantly changed (up or down >10 %) at 4 °C/24 h (bias is expressed as the mean±SEM; 13.37±21 %, p<0.05) and 22 °C/12 h (9.13±7.68 %, p<0.05) with the A method; and 4 °C/24 h (8.93±5.54 %, p<0.05), 22 °C/12 h (9.5±4.47 %, p<0.05) and −20 °C/3 h (12.03±4.8 %, p<0.05) with the C method.
Conclusions
After ACTH samples were centrifuged, the presence of plasma without removal did not affect the detection value, and the sample was stored at 4 °C for up to 24 h. There was a significant difference in the detection of ATCH when the sample was stored at −20 °C and thawed again (p<0.05).
Funder
Natural Science Foundation of Wuhan Science and Technology Bureau New Century Talent Program of Tianjin Medical University General Hospital Youth Incubation Fund of Tianjin Medical University General Hospital Tianjin Health Technology Project
Publisher
Walter de Gruyter GmbH
Subject
Biochemistry (medical),Clinical Biochemistry,Discrete Mathematics and Combinatorics
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