Nanomedical detection and downstream analysis of circulating tumor cells in head and neck patients
Author:
Künzel Julian1, Gribko Alena1, Lu Qiang1, Stauber Roland H.1, Wünsch Désirée1
Affiliation:
1. Nanobiomedicine Department/Department of Otorhinolaryngology-Head and Neck Surgery/ENT , University Medical Center Mainz , Langenbeckstrasse 1 , D-55131 Mainz , Germany
Abstract
Abstract
The establishment of novel biomarkers in liquid biopsies of cancer patients has come more into focus in prognostic and diagnostic research efforts. Due to their prognostic relevance disseminated tumor cells or circulating tumor cells are the subject of intensive research and are discussed as early diagnostic indicators for treatment failure and the formation of micrometastases. A potential association of this early-systemic tumor component with poor prognosis of cancer patients could be already demonstrated for various entities including breast, colon, lung, melanoma, ovarian and prostate cancers. Thus, the detection of circulating tumor cells seems to be also applicable for minimal-invasive monitoring of therapy progress in head and neck cancer patients. A major problem of the use in clinical routine is that circulating tumor cells could not be detected by modern imaging techniques. To overcome these limitations highly sensitive detection methods and techniques for their molecular characterization are urgently needed allowing mechanistic understanding and targeting of circulating tumor cells. Especially the medical application of nanotechnology (nanomedical methods) has made valuable contributions to the field. Here, we want to provide a comprehensive overview on (nanomedical) detection methods for circulating tumor cells and discuss their merits, pitfalls and future perspectives especially for head and neck solid squamous cell carcinoma (HNSCC) patients.
Publisher
Walter de Gruyter GmbH
Subject
Clinical Biochemistry,Molecular Biology,Biochemistry
Reference126 articles.
1. Alix-Panabieres, C. and Pantel, K. (2014). Challenges in circulating tumour cell research. Nat. Rev. Cancer 14, 623–631. 2. Alix-Panabieres, C., Rebillard, X., Brouillet, J.P., Barbotte, E., Iborra, F., Segui, B., Maudelonde, T., Jolivet-Reynaud, C., and Vendrell, J.P. (2005). Detection of circulating prostate-specific antigen-secreting cells in prostate cancer patients. Clin. Chem. 51, 1538–1541. 3. Alix-Panabieres, C., Bartkowiak, K., and Pantel, K. (2016). Functional studies on circulating and disseminated tumor cells in carcinoma patients. Mol. Oncol. 10, 443–449. 4. Balasubramanian, P., Yang, L., Lang, J.C., Jatana, K.R., Schuller, D., Agrawal, A., Zborowski, M., and Chalmers, J.J. (2009). Confocal images of circulating tumor cells obtained using a methodology and technology that removes normal cells. Mol. Pharm. 6, 1402–1408. 5. Balic, M., Rapp, N., Stanzer, S., Lin, H., Strutz, J., Szkandera, J., Daidone, M.G., Samonigg, H., Cote, R.J., and Dandachi, N. (2011). Novel immunofluorescence protocol for multimarker assessment of putative disseminating breast cancer stem cells. Appl Immunohistochem Mol. Morphol. 19, 33–40.
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