Preparation of [64Cu]Cu–NOTA complex as a potential renal PET imaging agent using 64Cu produced via the direct activation route
Author:
Patra Sourav12, Jadhav Sachin1, Shetty Priyalata12, Singh Khajan1, Rajeswari Ardhi1, Vimalnath K. V.1, Chakraborty Avik23, Chakravarty Rubel12, Chakraborty Sudipta12ORCID
Affiliation:
1. Radiopharmaceuticals Division , Bhabha Atomic Research Centre , Trombay , Mumbai 400085 , India 2. Homi Bhabha National Institute , Anushaktinagar , Mumbai 400094 , India 3. Radiation Medicine Centre , Bhabha Atomic Research Centre , Trombay , Mumbai 400085 , India
Abstract
Abstract
Glomerular filtration rate (GFR) could be determined more accurately using renal positron emission tomography (PET) than conventional gamma imaging. Copper-64 [T
½ = 12.7 h, E
β+ (max) = 653 keV, β+ branching ratio = 17.8 %, 1346 keV γ-photon (0.54 %), EC (43.8 %), β− emission (38.4 %)] in the form of its hydrophilic complex with 1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA) is proposed as a potent formulation for renal PET imaging. A lyophilized kit was developed for formulation of ∼370 MBq dose of [64Cu]Cu–NOTA complex in a facile single step process using 64Cu produced by thermal neutron activation in a research reactor. The complex could be synthesized with >99 % yield and retained its integrity even when challenged by apoferritin. The rapid accumulation of [64Cu]Cu–NOTA in the kidney and clearance through urinary path was demonstrated using PET/CT imaging and ex vivo biodistribution study carried out in healthy Wistar rats to elucidate its effectiveness as a renal PET-imaging agent.
Funder
Bhabha Atomic Research Centre
Publisher
Walter de Gruyter GmbH
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