Association of Permeability and Lipid Content of Membrane

Abstract

AbstractBackground:Rat skin and goatcul de sacare mostly used in optimization of formulations as the model of human skin andcul de sac.Aim:To explore the correlation between lipid content of rat skin and goatcul de sacand permeability.Materials and methods:Find out wavelength maximum for Sapat plus malam®, Ciplox eye ointment®and chloramphenicol eye caps and the standard curve was also derived.In vitrostudies using Cellophane®membrane andex vivostudies using rat skin or goatcul de sacof the formulations. Permeability coefficient, % dislodgeable dose, lag time, diffusion parameter, and partition coefficient were found for both studies after six and a half hours of penetration studies. Student’s unpaired t-test with equal variance was used to find any statistically significant difference in theex vivoandin vitrodiffusion transport studies at 95% level of confidence.Results:Permeability coefficient of Sapat plus malam®, Ciplox eye ointment®and chloramphenicol eye caps were 0.000316 ± 0.0000625, 0.00416 ± 0.0001, 0.0034 ± 0.00004 for Cellophane®membrane and 0.0001 ± 0.000001, 0.002254 ± 0.0002, 0.00303 ± 0.0001 forex vivomembrane in cm2/min, respectively. For all three formulations, there were calculated t values which were higher than tabulated t values at 95% of confidence level (P<0.05).Conclusion:Cellophane®membrane shows a better diffusion than rat skin or goatcul de sac. In the optimization of formulation, only Cellophane®membrane is advisable to use.